CD80 anticorps (APC)

N° du produit ABIN6657767

N° du produit (Fournisseur): 200-b26-n89

Cet anticorps anti-CD80 est un anticorps Hamster Monoclonal détectant CD80 dans FACS, IHC et IP. Adapté pour Souris.

Aperçu rapide pour CD80 anticorps (APC) (ABIN6657767)

Antigène: CD80

Reactivité: Souris

Hôte: Hamster

Clonalité: Monoclonal

Conjugué: Cet anticorp CD80 est conjugé à/à la APC

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Clone: 16-10A1

Détail du produit anti-CD80 anticorps (APC)

N° du produit (Fournisseur): 200-b26-n89

Fournisseur: Rockland

Fonction: CD80 Allophycocyanin Antibody

Réactivité croisée (Details): CD80 has cross reactivity with canine, cross reactivity from other sources has not been tested.

Purification: Allophycocyanin conjugated CD80 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD80.

Stérilité: Sterile filtered

Immunogène: Anti-CD80 Antibody (Monoclonal) was produced by repeated immunizations with CHO cell line transfected with mouse B7 (CD80).

Isotype: IgG

Labeling Ratio: 1-2

Information d'application

Indications d'application:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: 10 μL/10^6 cells (0.1 μg)

Commentaires:

Anti-CD80 is tested for FLOW and useful for Immunohistochemistry and Immunoprecipitation using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.09 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Date de péremption: 6 months

Détails sur CD80

Antigène: CD80

Autre désignation: CD80

Sujet:

Synonyms: T-lymphocyte activation antigen CD80, Activation B7-1 antigen, B7, CD80, Cd80

Background: CD80 is a 60 kD highly glycosylated protein. It is a member of the Ig superfamily and is also known as B7-1, B7, and Ly-53. CD80 is constitutively expressed on dendritic cells and monocytes/macrophages, and inducibly expressed on activated B and T cells. The ligation of CD28 on T cells with CD80 and CD86 (B7-2) on antigen presenting cells (such as dendritic cells, macrophages, and B cells) elicits co-stimulation of T cells resulting in enhanced cell activation, proliferation, and cytokine production. CD80 appears to be expressed later in the immune response than CD86. CD80 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Gene Name: Cd80

ID gène: 12519

NCBI Accession: NP_033985

UniProt: Q00609

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints